CellExplorer: a graphical user interface and a standardized pipeline for exploring and classifying single cells acquired using extracellular electrodes, like silicon probes or tetrodes. Please visit the dedicated website CellExplorer.org for more information.

CellExplorer

3D-printed recoverable metal microdrives

We have designed a metal microdrive and head cap systems for extracellular recordings in freely moving rodents, which make silicon probes recoverable and reusable. Please visit the dedicated website: buzsakilab.github.io/3d_print_designs/ for more information. Assembled metal microdrives are available for purchase from 3Dneuro (our partner in production).

Metal microdrive

In the Buzsaki Lab, we created a large database and public data repository with more than a thousand electrophysiological sessions (about 40TB of raw and spike- and LFP-processed data). The datasets are from freely moving rodents and include sleep-task-sleep sessions (3 to 24 hrs continuous recording sessions) in various brain structures, including metadata. The database contains electrophysiological recordings performed in freely moving rats and mice collected by investigators in the Buzsaki Lab over several years (a subset from head-fixed mice). Sessions have been collected with extracellular electrodes using high-channel-count silicon probes, with spike sorted single units, and intracellular and juxtacellular combined with extracellular electrodes. Several sessions include physiologically and optogenetically identified units. The sessions have been collected from various brain region pairs: the hippocampus, thalamus, amygdala, post-subiculum, septal region, and the entorhinal cortex, and various neocortical regions. In most behavioral tasks, the animals performed spatial behaviors (linear mazes and open fields), preceded and followed by long sleep sessions. Brain state classification is provided. Please visit the Buzsaki lab databank to learn more.

Public datasets

th-1 on CRCNS.org: Extracellular recordings from multi-site silicon probes in the anterior thalamus and subicular formation of freely moving mice. Internally organized mechanisms of the head direction sense. Peyrache A, Lacroix MM, Petersen PC, Buzsaki G, Nature Neuroscience. 2015

Data collection for Theta rhythm perturbation by focal cooling of the septal pacemaker in awake rats
Highlights
– Cooling the medial septum slowed down theta oscillations in the hippocampus
– The spatial representation in the hippocampus remained intact
– Choice errors increased in a spatial task
– Distance-time, but not a distance-theta phase, compression was altered

Databank link: https://buzsakilab.com/wp/projects/entry/4919/

figshare DOI (with data as well): https://doi.org/10.6084/m9.figshare.c.5264372

GitHub repositories

Please visit github.com/petersenpeter for full overview, including the KilosortWrapper, Phy plugins and a 3D printed base for head caps.